NOGO-A Neutralizing Immunoglobulins for the Treatment of Neurological Diseases

ABSTRACT

The present invention relates to antibodies to NOGO, pharmaceutical formulations containing them and to the use of such antibodies in the treatment and/or prophylaxis of neurological diseases/disorders.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.12/861,205, filed Aug. 23, 2010 which is a continuation of U.S.application Ser. No. 11/177,648, filed Jul. 6, 2005, now U.S. Pat. No.7,780,964, which is a continuation in part of International applicationNo. PCT/GB04/05325, filed Dec. 20, 2004, which claims priority fromUnited Kingdom application Nos. 0329711.6 and 0329684.5 both filed onDec. 22, 2003. The contents of all of the above-referenced applicationsare herein incorporated by reference.

FIELD OF THE INVENTION

The present invention relates to immunoglobulins, particularlyantibodies that bind to NOGO and neutralise the activity thereof,polynucleotides encoding such antibodies, pharmaceutical formulationscontaining said antibodies and to the use of such antibodies in thetreatment and/or prophylaxis of neurological diseases.

BACKGROUND OF THE INVENTION

Stroke is a major cause of death and disability in the Western World.There is no approved therapy for the treatment of stroke other thantissue plasminogen (t-PA) which has to be administered within 3 hours ofonset following a computer tomography (CT) scan to exclude haemorrhage.To date most therapeutic agents directed towards the treatment of acutestroke (i.e. neuroprotection), have predominantly involved targetingglutamate receptors and their down stream signalling pathways known tobe involved in acute cell death. However to date these strategies haveproved unsuccessful in clinical trials and are often associated withdose-limiting side effects (Hill & Hachinski, The Lancet, 352: (supplIII) 10-14 (1998)). Therefore there is a need for novel approachesdirected towards the amelioration of cell death following the cessationof blood flow. Neuroprotection is the ability of a treatment to preventor ameliorate neuronal cell loss in response to an insult or diseaseprocess. This maybe achieved by targeting the neurons directly orindirectly by preventing glial (including oligodendrocyte) cell loss.

Following the onset of stroke, some degree of spontaneous functionalrecovery is observed in many patients, suggesting that the brain has the(albeit limited) ability to repair and/or remodel following injury.Agents that have the potential to enhance this recovery may thereforeallow intervention to be made much later (potentially days) followingthe onset of cerebral ischaemia. Agents which are able to offer bothacute neuroprotection and enhance functional recovery may providesignificant advantages over current potential neuroprotectivestrategies.

Alzheimer's disease (AD) is characterised by the presence of twodiagnostic features of pathology. These are amyloid plaques andneurofibrillary tangles composed of aggregated beta-amyloid peptide(Aβ40 and Aβ42) and hyperphosphorylated tau respectively (Dawbarn &Allen 2001 Neurobiology of Alzheimer's Disease OUP).

A comprehensive study has shown a strong link in patients betweenbeta-amyloid accumulation and cognitive decline (Naslund et al, JAMA,Mar. 22/29, 2000, Vol. 283, No; 12, page 1571-1577). This is consistentwith genetic and epidemiological studies that suggest that somemutations in APP and presenilin genes can predispose to early onset AD,which mutations also enhance the levels of Aβ40 and Aβ42 peptide,including the ratio thereof.

Cleavage of the type I transmembrane amyloid precursor protein (APP) bytwo distinct proteases designated beta- and gamma-secretase is necessaryfor the formation of beta-amyloid peptide. The molecular identity ofbeta-secretase as the aspartyl-protease Asp2/BACE1 has been confirmed(Hussain et al Mol. Cell. NeuroSci. 16, 609-619 (2000); Vassar et al,Science (1999), Oct. 22; 286 (5440):735-741). The nature ofgamma-secretase remains the source of some debate and is likely toconsist of a high molecular weight complex consisting of at least thefollowing proteins: presenilins, Aph1, Pen2 and nicastrin (reviewed inMedina & Dotti Cell Signalling 2003 15(9):829-41).

The processing of APP within the CNS is likely to occur within a numberof cell-types including neurons, oligodendrocytes, astrocytes andmicroglia. While the overall rate of APP processing in these cells willbe influenced by the relative level of expression of APP, BACE1/Asp2,presenilin-1 and -2, Aph1, Pen2 and nicastrin.

Furthermore, additional factors regulating the subcellular location ofAPP can also influence its processing as shown by the finding thatmutation of the YENP motif in the APP cytoplasmic domain which blocksits endocytosis reduces beta-amyloid production (Perez et al 1999 J BiolChem 274 (27) 18851-6). Retention of the APP-beta-CTF in the ER by theaddition of the KKQN retention motif is sufficient to reduce amyloidproduction in transfected cells (Maltese et al 2001 J Biol Chem 276 (23)20267-20279). Conversely, elevation of endocytosis, by overexpression ofRabS is sufficient to elevate amyloid secretion from transfected cells(Grbovic et al 2003 J Biol Chem 278 (33) 31261-31268).

Consistent with these findings further studies have shown that reductionof cellular cholesterol levels (a well known risk factor for AD) reducedbeta-amyloid formation. This change was dependent on altered endocytosisas demonstrated by the use of the dominant negative dynamin mutants(K44A) and overexpression of the RabS GTPase activating protein RN-Tre(Ehehalt et al 2003 J Cell Biol 160 (1) 113-123).

Cholesterol rich microdomains or rafts are also an important cellularsite of beta-amyloid production and APP, BACE1 and components of thegamma-secretase complex have all been shown to transiently reside withinrafts. Antibody cross-linking of APP and BACE1 towards cholesterol richrafts was able to elevate beta-amyloid production (Ehehalt et al 2003 JCell Biol 160 (1) 113-123). Expression of GPI-anchored BACE1, which isexclusively targeted to lipid rafts, is similarly able to elevate APPcleavage and beta-amyloid production (Cordy et al 2003 PNAS 100(20)11735-11740).

The mechanisms underlying functional recovery are currently unknown. Thesprouting of injured or non-injured axons has been proposed as onepossible mechanism. However, although in vivo studies have shown thattreatment of spinal cord injury or stroke with neurotrophic factorsresults in enhanced functional recovery and a degree of axonalsprouting, these do not prove a direct link between the degree of axonalsprouting and extent of functional recovery (Jakeman, et al. 1998, Exp.Neurol. 154: 170-184, Kawamata et al. 1997, Proc Natl Acad. Sci. USA.,94:8179-8184, Ribotta, et al. 2000, J. Neurosci. 20: 5144-5152).Furthermore, axonal sprouting requires a viable neuron. In diseases suchas stroke which is associated with extensive cell death, enhancement offunctional recovery offered by a given agent post stroke may thereforebe through mechanisms other than axonal sprouting such asdifferentiation of endogenous stem cells, activation of redundantpathways, changes in receptor distribution or excitability of neurons orglia (Fawcett & Asher, 1999, Brain Res. Bulletin, 49: 377-391, Horner &Gage, 2000, Nature 407 963-970).

The limited ability of the central nervous system (CNS) to repairfollowing injury is thought in part to be due to molecules within theCNS environment that have an inhibitory effect on axonal sprouting(neurite outgrowth). CNS myelin is thought to contain inhibitorymolecules (Schwab M E and Caroni P (1988) J. Neurosci. 8, 2381-2193).Two myelin proteins, myelin-associated glycoprotein (MAG) and NOGO havebeen cloned and identified as putative inhibitors of neurite outgrowth(Sato S. et al (1989) Biochem. Biophys. Res. Comm. 163, 1473-1480;McKerracher L et al (1994) Neuron 13, 805-811; Mukhopadhyay G et al(1994) Neuron 13, 757-767; Torigoe K and Lundborg G (1997) Exp.Neurology 150, 254-262; Schafer et al (1996) Neuron 16, 1107-1113;WO9522344; WO9701352; Prinjha R et al (2000) Nature 403, 383-384; Chen MS et al (2000) Nature 403, 434-439; GrandPre T et al (2000) Nature 403,439-444; US005250414A; WO200005364A1; WO0031235).

Three forms of human NOGO have been identified: NOGO-A having 1192 aminoacid residues (GenBank accession no. AJ251383); NOGO-B, a splice variantwhich lacks residues 186 to 1004 in the putative extracellular domain(GenBank accession no. AJ251384) and a shorter splice variant, NOGO-C,which also lacks residues 186 to 1004 and also has smaller, alternativeamino terminal domain (GenBank accession no. AJ251385) (Prinjha et al(2000) supra).

Inhibition of the CNS inhibitory proteins such as NOGO may provide atherapeutic means to ameliorate neuronal damage and promote neuronalrepair and growth thereby potentially assisting recovery from neuronalinjury such as that sustained in stroke. Examples of such NOGOinhibitors may include small molecules, peptides and antibodies.

Antibodies typically comprise two heavy chains linked together bydisulphide bonds and two light chains. Each light chain is linked to arespective heavy chain by disulphide bonds. Each heavy chain has at oneend a variable domain followed by a number of constant domains. Eachlight chain has a variable domain at one end and a constant domain atits other end. The light chain variable domain is aligned with thevariable domain of the heavy chain. The light chain constant domain isaligned with the first constant domain of the heavy chain. The constantdomains in the light and heavy chains are not involved directly inbinding the antibody to antigen.

The variable domains of each pair of light and heavy chains form theantigen binding site. The variable domains on the light and heavy chainshave the same general structure and each domain comprises a framework offour regions, whose sequences are relatively conserved, connected bythree complementarity determining regions (CDRs) often referred to ashypervariable regions. The four framework regions largely adopt abeta-sheet conformation and the CDRs form loops connecting, and in somecases forming part of, the beta-sheet structure. The CDRs are held inclose proximity by the framework regions and, with the CDRs from theother domain, contribute to the formation of the antigen binding site.CDRs and framework regions of antibodies may be determined by referenceto Kabat et al (“Sequences of proteins of immunological interest” USDept. of Health and Human Services, US Government Printing Office,1987).

It has been reported that a murine monoclonal antibody, IN-1, that wasraised against NI-220/250, a myelin protein which is a potent inhibitorof neurite growth (and subsequently shown to be fragment of NOGO-A),promotes axonal regeneration (Caroni, P and Schwab, M E (1988) Neuron 185-96; Schnell, L and Schwab, M E (1990) Nature 343 269-272; Bregman, BS et al (1995) Nature 378 498-501 and Thallmair, M et al (1998) NatureNeuroscience 1 124-131). It has also been reported that NOGO-A is theantigen for IN-1 (Chen et al (2000) Nature 403 434-439). Administrationof IN-1 Fab fragment or humanised IN-1 to rats that have undergonespinal cord transection, enhanced recovery (Fiedler, M et al (2002)Protein Eng 15 931-941; Brosamle, C et al (2000) J. Neuroscience 208061-8068). However to date there is no evidence in the literature tosuggest that IN-1, or its humanised form, can bind and inhibit humanNOGO-A, a necessary requirement for a monoclonal antibody to be usefulin the therapeutic treatment of NOGO-mediated diseases and disorderssuch as stroke and neurodegenerative diseases in humans.

Monoclonal antibodies which bind to NOGO are described in WO 04/052932and WO2005028508. WO 04/052932 discloses a murine antibody 11C7 whichbinds to certain forms of human NOGO with high affinity.

It is desirable to isolate and develop further therapeutically usefulmonoclonal antibodies that bind to, and inhibit the activity of, humanNOGO. The process of neurodegeneration underlies many neurologicaldiseases/disorders including, but not limited to, acute diseases such asstroke (ischemic or haemorrhagic), traumatic brain injury and spinalcord injury as well as chronic diseases including Alzheimer's disease,fronto-temporal dementias (tauopathies), peripheral neuropathy,Parkinson's disease, Creutzfeldt-Jakob disease (CJD), Schizophrenia,amyotrophic lateral sclerosis (ALS), multiple sclerosis, Huntington'sdisease, multiple sclerosis and inclusion body myositis. Consequently ananti-NOGO monoclonal antibody may be useful in the treatment of thesediseases/disorders. Such antibodies for the treatment of the abovementioned disease/disorders are provided by the present invention anddescribed in detail below.

The unpublished patent application, with the International PatentApplication number PCT/GB2004/005325, also discloses high affinitymonoclonal antibodies, including a murine monoclonal antibody 2A10, anda humanised variant thereof H1L11.

SUMMARY OF THE INVENTION

The present invention provides a number of monoclonal antibodies thatbind to human NOGO. The present application gives reference to many SEQID numbers, which are summarised in Table 12, with the actual sequencesfollowing that table towards the end of this document.

The murine antibody 2A10 binds to human NOGO with high affinity, andbinds to the form of NOGO which is expressed by human cell lines withhigh affinity. 2A10 has been humanised successfully in the past (H1L11which comprises the heavy chain variable region H1 (SEQ ID NO. 77) andthe light chain variable region L11 (SEQ ID NO. 78)). The presentinvention provides additional humanised monoclonal antibodies whichretain a high proportion of the affinity of the donor antibody (2A10) tohuman NOGO. In particular the antibodies of the present invention have ahigh affinity to human NOGO both in recombinant form as expressed inbacterial cells (such as E. Coli), and also in the form that it isexpressed by a human neuroblastoma cell line (for example the humanneuroblastoma cell line IMR32).

For the purposes of providing humanised variants of 2A10, the presentinventors have identified a number of key amino acid residues in theframework sequence of the 2A10 variable regions which are believed to beimportant in optimal retention of binding affinity to human NOGO. The2A10 heavy chain variable region (VH) is provided in SEQ ID NO. 7; the2A10 light chain variable region (VL) is provided in SEQ ID NO. 8.Chimeric heavy and light chains comprising the murine variable regionsand human constant regions are provided in SEQ ID NOs 9 and 10respectively (the combination of the two chimeric chains is termedHcLc). The skilled reader will understand that SEQ ID NOs 9 and 10represent the heavy chain or light chains prior to any processing (e.g.host cell mediated processing) for removal of a signal sequence.Typically the processed forms of the antibody chains will begin atposition 20 (after the removal of the signal sequence (residues 1-19)which corresponds to SEQ ID NO. 75).

TABLE 1 CDRs of the 2A10 heavy chain are: CDR According to Kabat H1SYWMH (SEQ ID NO: 1) H2 NINPSNGGTNYNEKFKS (SEQ ID NO: 2) H3 GQGY (SEQ IDNO: 3)

TABLE 2 CDRs of 2A10 light chain: CDR According to Kabat L1RSSKSLLYKDGKTYLN (SEQ ID NO: 4) L2 LMSTRAS (SEQ ID NO: 5) L3 QQLVEYPLT(SEQ ID NO: 6)

The CDRs were identified according to Kabat (Kabat et al. (1991)“Sequences of proteins of immunological interest”; Fifth Edition; USDepartment of Health and Human Services; NIH publication No 91-3242).CDRs preferably are as defined by Kabat but following the principles ofprotein structure and folding as defined by Chothia and Lesk, (Chothiaet al., (1989) “Conformations of immunoglobulin hypervariable regions”;Nature 342, p 877-883) it will be appreciated that additional residuesmay also be considered to be part of the antigen binding region and arethus encompassed by the present invention.

The VH and VL domains H1 and L11 have been previously described inPCT/GB2004/005325, and are a result of the CDRs mentioned in tables 1and 2 being grafted into human variable regions with high homology tothe 2A10 donor antibody, each grafted construct further comprising backmutations in kabat positions 93 and 94 (for the H1 VH) or 4, 45 and 46(for L11 VL).

The 2A10 antibody is capable of binding to human NOGO, and also binds toMarmoset and Rat NOGO, and it is believed that the new humanisedantibodies of the present invention will retain that property. Thesequence of Marmoset NOGO-A fragment is given in SEQ ID NO. 113.

Heavy Chain Variable Region (VH)

In one aspect of the present invention the antibodies comprise a heavychain variable region having the amino acid sequence of SEQ ID NO. 77(H1 variable region) further comprising a number of substitutions at oneor more of positions 12, 20, 38, 40, 48, 67, 68, 70, 72, 74, 76, 79 and91; wherein each substituted amino acid residue is replaced with theamino acid residue at the equivalent position in SEQ ID NO 7 (the heavychain variable region of the donor antibody 2A10) and the number ofsubstitutions is between 1 and 13. In other embodiments the number ofsubstitutions is 1, or 2, or 3, or 4, or 5, or 6, or 7, or 8, or 9, or10, or 11, or 12, or 13. In other embodiments the number ofsubstitutions is between 2 and 13, or 3 and 13, or 4 and 13, or 5 and13, or 6 and 13, or 7 and 13, or 8 and 13, or 9 and 13, or 10 and 13 or11 and 13, or 12 and 13.

In this context the substitutions that are described are equivalent inconcept to “back-mutations” where the human framework amino acidresidues in specific positions within the H1 sequence are back-mutatedto the amino acid residues in the equivalent position within the 2A10donor antibody sequence.

Unless specifically stated otherwise, when a numerical position of anamino acid residue found within a specific sequence is mentioned in thisdocument, for example “position 12”, it is intended that the skilledreader assigns the first amino acid in the sequence the position “1” andcounts from position one and identifies the amino acid which is in thedesired position, in this example the twelfth amino acid residue in thesequence. The skilled reader will notice that this numbering system doesnot correspond with the Kabat numbering system which is often used foramino acid positions within antibody sequences. The following table(Table 3) illustrates the substitutions/back-mutations of the presentinvention and gives their numerical positions and the Kabat number forthat numerical position:

TABLE 3 Kabat Corresponding Human framework number of residue in residueof H1 Numerical that numerical murine 2A10 (SEQ ID NO 77) Positionposition (SEQ ID NO 7) K 12 12 V V 20 20 L R 38 38 K A 40 40 R M 48 48 IR 67 66 K V 68 67 A M 70 69 L R 72 71 V T 74 73 K T 76 75 S V 79 78 A T91 87 S

With reference to Table 3, in one embodiment the monoclonal antibodiesof the present invention comprise the substitution/backmutation atposition 79 to form antibodies of “Class A”.

For optimal binding affinity, it has been found that the pair of aminoacid residues in positions 48 and 68, should be I and A respectively (asthey exist in 2A10) or M and V respectively (as they exist in H1).Accordingly, again with reference to Table 3, in another embodiment,“Class B”, the monoclonal antibody comprises the substitution atpositions 79, 48 and 68.

In another embodiment (“Class C”) the monoclonal antibodies of “Class A”or “Class B” further comprise a substitution at positions 40 and/or 67.

In another embodiment the “Class C” monoclonal antibodies of the presentinvention further comprise a substitution at positions 38, 72 or 70 toform “Class D” antibodies.

In another embodiment the “Class D” monoclonal antibodies furthercomprise substitutions at one or more of positions 12, 20, 74, 76 or 91(“Class E”).

The following table includes details of 20 different heavy chainvariable (VH) regions which may form part of the antibodies of thepresent invention. Each of the disclosed VH is based on the H1 VH (SEQID NO. 77) further comprising the substitutions mentioned in the table(Table 4) where the H1 residue at the relevant position is substitutedwith the 2A10 residue at that position (in the table, “−” means thatthere is no substitution in that position, and so the residue remains asin the sequence of H1):

TABLE 4 Numerical Residue No. 12 20 38 40 48 67 68 70 72 74 76 79 91Kabat No. 12 20 38 40 48 66 67 69 71 73 75 78 87 2A10 New VH V L K R I KA L V K S A S (SEQ ID H1 NO. X) K V R A M R V M R T T V T H5 (11) — — —— — — — — — — — A — H6 (12) — — — — I — A — — — — A — H700 (13) — — — —I — A L V K — A — H8 — — — — I — A L V — — A — H9 — L — — I — A L V K —A — H10 — L K — I — A L V K — A — H12 — — — — I — A L — K — — — H13 — —— — I — A — V — — A — H14 (14) V L K — I — A L — — — A — H15 (15) V L KR I K A L V — S A S H16 (16) — — K R I K A L V K — A — H17 (17) V L K RI K A L V K — A — H18 (18) V L K R I K A L V K S A S H19 (85) — — — R I— A — — — — A — H20 (86) — — — — I K A — — — — A — H21 (87) — — — R I KA — — — — A — H22 (88) — — K R I K A — — — — A — H23 (89) — — K R I K A— V — — A — H24 (90) — — K R I K A L V — — A — H25 (91) — — — R — — — —— — — A —

Accordingly there is provided a monoclonal antibody comprising the heavychain variable region having a sequence given in any one of SEQ ID NOs:11-18 or 85-91. In another embodiment there is provided a monoclonalantibody comprising a heavy chain having a sequence given in any one ofSEQ ID NOs: 26-33 or 92-98.

In a particular embodiment the antibody comprises the VH regionsprovided in SEQ ID NOs 11, 12, 16, 18, 85, 86, 87 or 91 or the heavychains provided in SEQ ID NOs 26, 27, 31, 33, 92, 93, 94 or 98.

Light Chain Variable Region

In one aspect of the present invention the antibodies comprise a lightchain variable region having the amino acid sequence of SEQ ID NO. 20(L13 variable region) optionally further comprising a number ofsubstitutions at one or more of positions 4, 7, 11, 19, 42, 64 and 70;wherein each substituted amino acid residue is replaced with the aminoacid residue at the equivalent position in SEQ ID NO. 8 (the light chainvariable region of the donor antibody 2A10) and the number ofsubstitutions is between 0 and 7. In other embodiments the number ofsubstitutions is 1, or 2, or 3, or 4, or 5, or 6, or 7. In otherembodiments the number of substitutions is between 2 and 7, or 3 and 7,or 4 and 7, or 5 and 7, or 6 and 7.

The following table (Table 5) illustrates thesubstitutions/back-mutations of the present invention and gives theirnumerical positions and the Kabat number for that numerical position:

TABLE 5 Human framework Corresponding residue of L13 Numerical Kabatresidue in murine 2A10 (SEQ ID NO 20) Position number (SEQ ID NO 8) M 44 I S 7 7 D L 11 11 N A 19 19 V Q 42 37 L P 64 59 S G 70 65 S

With reference to Table 5, in another embodiment, “Class X”, themonoclonal antibodies of the present invention comprise a VL regionhaving the substitution/backmutation at positions 11 and 19. In anotherembodiment “Class Y” the monoclonal antibodies of “Class X” furthercomprise the substitution in position 42. In a further embodiment themonoclonal antibodies of “Class X” or “Class Y” further comprise a backmutation in positions 7, 64 or 70 to form “Class Z”.

Again with reference to Table 5, in another embodiment the monoclonalantibodies of the present invention comprise a VL region having asubstitution at position 4 (corresponding to L11 (SEQ ID NO. 78).

The following table (Table 6) includes details of seven different lightchain variable (VL) regions which may form part of the antibodies of thepresent invention. Each of the disclosed VL is, or is based on, the L13VL (SEQ ID NO. 20) optionally further comprising the substitutionsmentioned in the table where the L13 residue at the relevant position issubstituted with the 2A10 residue at that position (in the table, “−”means that there is no substitution in that position, and so the residueremains as in the sequence of L13):

TABLE 6 Numerical Residue No. 4 7 11 19 42 64 70 Kabat No. 4 7 11 19 3759 65 2A10 New VL I D N V L S S (SEQ ID L13 NO. X) M S L A Q P G L11(78) I — — — — — — L13 (20) — — — — — — — L14 (21) — — — — L — — L15(22) — — — — L S — L16 (23) — — N V L — — L17 (24) — D — — — S S L18(25) — D N V L S S

Accordingly there is provided a monoclonal antibody comprising the lightchain variable region having a sequence given in any one of SEQ ID NOs:20-25 or 78. In another embodiment there is provided a monoclonalantibody comprising a light chain having a sequence given in any one ofSEQ ID NOs: 35-40 or 80.

Alternatively there is provided a monoclonal antibody comprising thelight chain variable region having the sequence given in SEQ ID NO. 19,or a light chain having the sequence given in SEQ ID NO. 34.

In a particular embodiment the antibody comprises the VL regionsprovided in SEQ ID NOs 20, 23 and 25 or the light chains provided in SEQID NOs 35, 38 and 40.

Specific Combinations of Heavy and Light Chain Variable Regions

The antibodies of the present invention comprise a heavy chain variableregion and a light chain variable region.

In one embodiment the antibody comprises:

-   -   (a) A heavy chain variable region having the amino acid sequence        of SEQ ID NO. 77 (H1 variable region) further comprising a        number of substitutions at one or more of positions 12, 20, 38,        40, 48, 67, 68, 70, 72, 74, 76, 79 and 91; wherein each        substituted amino acid residue is replaced with the amino acid        residue at the equivalent position in SEQ ID NO 7 (the heavy        chain variable region of the donor antibody 2A10) and the number        of substitutions is between 1 and 13. In other embodiments the        number of substitutions is 1, or 2, or 3, or 4, or 5, or 6, or        7, or 8, or 9, or 10, or 11, or 12, or 13; and    -   (b) a light chain variable region selected from SEQ ID NOs 20-25        or 78.

Particular embodiments are antibodies comprising the followingcombinations of heavy and light chain variable regions: H1L13 (SEQ ID77+SEQ ID 20), H5L13 (SEQ ID 11+SEQ ID 20), H6L13 (SEQ ID 12+SEQ ID 20),H14L13 (SEQ ID 14+SEQ ID 20), H15L13 (SEQ ID 15+SEQ ID 20), H16L13 (SEQID 16+SEQ ID 20), H17L13 (SEQ ID 17+SEQ ID 20), H18L13 (SEQ ID 18+SEQ ID20), H19L13 (SEQ ID 85+SEQ ID 20), H20L13 (SEQ ID 86+SEQ ID 20), H21L13(SEQ ID 87+SEQ ID 20), H22L13 (SEQ ID 88+SEQ ID 20), H23L13 (SEQ ID89+SEQ ID 20), H24L13 (SEQ ID 90+SEQ ID 20), H25L13 (SEQ ID 91+SEQ ID20), H700L13 (SEQ ID 13+SEQ ID 20), H1L16 (SEQ ID 77+SEQ ID 23), H5L16(SEQ ID 11+SEQ ID 23), H6L16 (SEQ ID 12+SEQ ID 23), H14L16 (SEQ ID14+SEQ ID 23), H15L16 (SEQ ID 15+SEQ ID 23), H16L16 (SEQ ID 16+SEQ ID23), H17L16 (SEQ ID 17+SEQ ID 23), H18L16 (SEQ ID 18+SEQ ID 23), H19L16(SEQ ID 85+SEQ ID 23), H20L16 (SEQ ID 86+SEQ ID 23), H21L16 (SEQ ID87+SEQ ID 23), H22L16 (SEQ ID 88+SEQ ID 23), H23L16 (SEQ ID 89+SEQ ID23), H24L16 (SEQ ID 90+SEQ ID 23), H25L16 (SEQ ID 91+SEQ ID 23), H700L16(SEQ ID 13+SEQ ID 23), H1L18 (SEQ ID 77+SEQ ID 25), H5L18 (SEQ ID 11+SEQID 25), H6L18 (SEQ ID 12+SEQ ID 25), H14L18 (SEQ ID 14+SEQ ID 25),H15L18 (SEQ ID 15+SEQ ID 25), H16L18 (SEQ ID 16+SEQ ID 25), H17L18 (SEQID 17+SEQ ID 25), H18L18 (SEQ ID 18+SEQ ID 25), H19L18 (SEQ ID 85+SEQ ID25), H20L18 (SEQ ID 86+SEQ ID 25), H21L18 (SEQ ID 87+SEQ ID 25), H22L18(SEQ ID 88+SEQ ID 25), H23L18 (SEQ ID 89+SEQ ID 25), H24L18 (SEQ ID90+SEQ ID 25), H25L18 (SEQ ID 91+SEQ ID 25), H700L18 (SEQ ID 13+SEQ ID25).

Other embodiments are antibodies comprising the following combinationsof heavy and light chain variable regions: H1L14 (SEQ ID 77+SEQ ID 21),H5L14 (SEQ ID 11+SEQ ID 21), H6L14 (SEQ ID 12+SEQ ID 21), H14L14 (SEQ ID14+SEQ ID 21), H15L14 (SEQ ID 15+SEQ ID 21), H16L14 (SEQ ID 16+SEQ ID21), H17L14 (SEQ ID 17+SEQ ID 21), H18L14 (SEQ ID 18+SEQ ID 21), H19L14(SEQ ID 85+SEQ ID 21), H20L14 (SEQ ID 86+SEQ ID 21), H21L14 (SEQ ID87+SEQ ID 21), H22L14 (SEQ ID 88+SEQ ID 21), H23L14 (SEQ ID 89+SEQ ID21), H24L14 (SEQ ID 90+SEQ ID 21), H25L14 (SEQ ID 91+SEQ ID 21), H700L14(SEQ ID 13+SEQ ID 21), H1L15 (SEQ ID 77+SEQ ID 22), H5L15 (SEQ ID 11+SEQID 22), H6L15 (SEQ ID 12+SEQ ID 22), H14L15 (SEQ ID 14+SEQ ID 22),H15L15 (SEQ ID 15+SEQ ID 22), H16L15 (SEQ ID 16+SEQ ID 22), H17L15 (SEQID 17+SEQ ID 22), H18L15 (SEQ ID 18+SEQ ID 22), H19L15 (SEQ ID 85+SEQ ID22), H20L15 (SEQ ID 86+SEQ ID 22), H21L15 (SEQ ID 87+SEQ ID 22), H22L15(SEQ ID 88+SEQ ID 22), H23L15 (SEQ ID 89+SEQ ID 22), H24L15 (SEQ ID90+SEQ ID 22), H25L15 (SEQ ID 91+SEQ ID 22), H700L15 (SEQ ID 13+SEQ ID22), H1L17 (SEQ ID 77+SEQ ID 24), H5L17 (SEQ ID 11+SEQ ID 24), H6L17(SEQ ID 12+SEQ ID 24), H14L17 (SEQ ID 14+SEQ ID 24), H15L17 (SEQ ID15+SEQ ID 24), H16L17 (SEQ ID 16+SEQ ID 24), H17L17 (SEQ ID 17+SEQ ID24), H18L17 (SEQ ID 18+SEQ ID 24), H19L17 (SEQ ID 85+SEQ ID 24), H20L17(SEQ ID 86+SEQ ID 24), H21L17 (SEQ ID 87+SEQ ID 24), H22L17 (SEQ ID88+SEQ ID 24), H23L17 (SEQ ID 89+SEQ ID 24), H24L17 (SEQ ID 90+SEQ ID24), H25L17 (SEQ ID 91+SEQ ID 24), H700L17 (SEQ ID 13+SEQ ID 24).

Further embodiments are antibodies comprising the following combinationsof heavy and light chain variable regions: H1L6 (SEQ ID 77+SEQ ID 19),H5L6 (SEQ ID 11+SEQ ID 19), H6L6 (SEQ ID 12+SEQ ID 19), H14L6 (SEQ ID14+SEQ ID 19), H15L6 (SEQ ID 15+SEQ ID 19), H16L6 (SEQ ID 16+SEQ ID 19),H17L6 (SEQ ID 17+SEQ ID 19), H18L6 (SEQ ID 18+SEQ ID 19), H19L6 (SEQ ID85+SEQ ID 19), H20L6 (SEQ ID 86+SEQ ID 19), H21L6 (SEQ ID 87+SEQ ID 19),H22L6 (SEQ ID 88+SEQ ID 19), H23L6 (SEQ ID 89+SEQ ID 19), H24L6 (SEQ ID90+SEQ ID 19), H25L6 (SEQ ID 91+SEQ ID 19), H700L6 (SEQ ID 13+SEQ ID19), H5L11 (SEQ ID 11+SEQ ID 78), H6L11 (SEQ ID 12+SEQ ID 78), H14L11(SEQ ID 14+SEQ ID 78), H15L11 (SEQ ID 15+SEQ ID 78), H16L11 (SEQ ID16+SEQ ID 78), H17L11 (SEQ ID 17+SEQ ID 78), H18L11 (SEQ ID 18+SEQ ID78), H19L11 (SEQ ID 85+SEQ ID 78), H20L11 (SEQ ID 86+SEQ ID 78), H21L11(SEQ ID 87+SEQ ID 78), H22L11 (SEQ ID 88+SEQ ID 78), H23L11 (SEQ ID89+SEQ ID 78), H24L11 (SEQ ID 90+SEQ ID 78), H25L11 (SEQ ID 91+SEQ ID78), H700L11 (SEQ ID 13+SEQ ID 78).

Whole Antibodies

Further, the invention also provides a humanised antibody which binds toand neutralises NOGO, preferably human NOGO, more preferably humanNOGO-A. More specifically there is provided a humanised antibodycomprising a heavy chain variable region as described herein and a lightchain variable region as described herein.

The humanised antibodies of the present invention bind to human NOGOwith a comparable affinity to that of the murine donor antibody 2A10. Inone embodiment the binding of the antibody of the present invention toNOGO has an affinity constant (KD, as measured by BiaCore techniques)within 10 fold of 2A10, and in another embodiment the affinity constantis within three or two fold of 2A10. In another embodiment the affinityconstant is within three or two fold of that of 2A10 and the off-rate(kd) is within 10 fold, or three fold, or two fold of 2A10. The methodof measuring the affinity constant and the off-rate of the antibodyshould be clear to the skilled reader, however the kinetic analysisBiaCore method given in Example 5 of this document is illustrative inthis regard. For example, the affinity constant and the off-rate of 2A10as measured by BiaCore kinetic analysis is about commonly in the regionof 1 nM and 1.84×10⁻³ (kd 1/s) respectively; in the same assay theantibodies of one embodiment of the present invention will have anaffinity constant of less than 8-10 nM and 1.84×10⁻².

In typical embodiments, the antibodies of the invention are of the IgGclass, more typically human IgG1 or IgG4, with a K type human lightchain.

A further aspect of the invention provides a pharmaceutical compositioncomprising an anti-NOGO antibody of the present invention or functionalfragment thereof together with a pharmaceutically acceptable diluent orcarrier.

In a further aspect, the present invention provides a method oftreatment or prophylaxis of stroke (particularly ischemic stroke) andother neurological diseases, in particular Alzheimer's disease, in ahuman which comprises administering to said human in need thereof aneffective amount of an anti-NOGO antibody of the invention or functionalfragments thereof.

In another aspect, the invention provides the use of an anti-NOGOantibody of the invention or a functional fragment thereof in thepreparation of a medicament for treatment or prophylaxis of stroke(particularly ischemic stroke) and other neurological diseases, inparticular Alzheimer's disease.

In a further aspect, the present invention provides a method ofinhibiting neurodegeneration and/or promoting functional recovery in ahuman patient afflicted with, or at risk of developing, a stroke(particularly ischemic stroke) or other neurological disease, inparticular Alzheimer's disease, which comprises administering to saidhuman in need thereof an effective amount of an anti-NOGO antibody ofthe invention or a functional fragment thereof.

In a yet further aspect, the invention provides the use of an anti-NOGOantibody of the invention or a functional fragment thereof in thepreparation of a medicament for inhibiting neurodegeneration and/orpromoting functional recovery in a human patient afflicted with, or atrisk of developing, a stroke and other neurological disease, inparticular Alzheimer's disease.

Other aspects and advantages of the present invention are describedfurther in the detailed description and the preferred embodimentsthereof.

In one embodiment the full length antibodies are those comprising thelight chains of SEQ ID NOs 34-40 and the heavy chains of SEQ ID NOs92-98; and in particular the light chains of SEQ ID NOs 35, 38 or 40 andheavy chains of SEQ ID NOs 92, 93, 94 or 98. It will be apparent tothose skilled in the art that all of the sequences given for the fulllength antibody chains in Table 7 and 12 (and appended sequences)represent the heavy chain or light chains prior to any processing (e.g.host cell mediated processing) for removal of a signal sequence.Typically the processed forms of the antibody chains will begin atposition 20 (after the removal of the signal sequence (residues 1-19)which corresponds to SEQ ID NO. 75). The present invention provides theantibodies having the polypeptide sequences listed (after removal of thefirst 19 amino acids of the signal sequence), and also provides theantibodies in the form in which they are produced and purified from hostcells expressing the polynucleotides encoding the heavy and light chain.

TABLE 7 Specific full length antibodies include: Antibody Light ChainHeavy Chain H5L13 FL SEQ ID NO. 35 SEQ ID NO. 26 H6L13 FL SEQ ID NO. 35SEQ ID NO. 27 H19L13 FL SEQ ID NO. 35 SEQ ID NO. 92 H20L13 FL SEQ ID NO.35 SEQ ID NO. 93 H21L13 FL SEQ ID NO. 35 SEQ ID NO. 94 H25L13 FL SEQ IDNO. 35 SEQ ID NO. 98 H16L16 FL SEQ ID NO. 38 SEQ ID NO. 31 H19L16 FL SEQID NO. 38 SEQ ID NO. 92 H20L16 FL SEQ ID NO. 38 SEQ ID NO. 93 H21L16 FLSEQ ID NO. 38 SEQ ID NO. 94 H25L16 FL SEQ ID NO. 38 SEQ ID NO. 98 H16L18FL SEQ ID NO. 40 SEQ ID NO. 31 H18L16 FL SEQ ID NO. 38 SEQ ID NO. 33H19L18 FL SEQ ID NO. 40 SEQ ID NO. 92 H20L18 FL SEQ ID NO. 40 SEQ ID NO.93 H21L18 FL SEQ ID NO. 40 SEQ ID NO. 94 H25L18 FL SEQ ID NO. 40 SEQ IDNO. 98

Alternatively the substitutions mentioned above, being the backmutations to the exact amino residue found in the equivalent positionwithin the donor 2A10 murine sequence, may be any substitution to anamino acid which is a conservative substitution of the exact residuefound in the equivalent position within the donor 2A10 murine sequence.The term “conservative substitution” is clear to the skilled reader, andincludes for instance a substitution of an amino acid with another aminoacid residue having a similar physical, chemical or structural propertysuch as pH, charge, hydrophobicity, aromaticity etc.

DESCRIPTION OF THE FIGURES

FIGS. 1 A and B, ELISA data for monoclonal antibody supernatants bindingto recombinant human NOGO.

FIGS. 2 A and B, ELISA data for purified monoclonal antibody binding torecombinant human NOGO.

FIGS. 3 A and B, ELISA data for monoclonal antibody supernatants bindingto recombinant human NOGO.

FIGS. 4 A and B, ELISA data for monoclonal antibody supernatants bindingto recombinant human NOGO.

FIGS. 5 A and B, ELISA data for monoclonal antibody supernatants bindingto recombinant human NOGO.

FIG. 6, A to F, FACS data for purified antibody binding to human NOGOexpressed by human neuroblastoma cells.

FIG. 7, Competition ELISA results

DETAILED DESCRIPTION OF THE INVENTION

Antibodies of the invention typically have the structure of a naturalantibody or functional fragment thereof. The antibody may thereforecomprise a full length antibody, a (Fab′)₂ fragment, a Fab fragment, alight chain dimer or a heavy chain dimer. The antibody may be an IgG1,IgG2, IgG3, or IgG4; or IgM; IgA, IgE or IgD or a modified variantthereof. The constant domain of the antibody heavy chain may be selectedaccordingly. The light chain constant domain may be a kappa or lambdaconstant domain. Furthermore, the antibody may comprise modifications ofall classes eg. IgG dimers, Fc mutants that no longer bind Fc receptorsor mediate Clq binding. The antibody may also be a chimeric antibody ofthe type described in WO86/01533 which comprises an antigen bindingregion and a non-immunoglobulin region. The antigen binding region is anantibody light chain variable domain or heavy chain variable domain.Typically the antigen binding region comprises both light and heavychain variable domains. The non-immunoglobulin region is fused at its Cterminus to the antigen binding region. The non-immunoglobulin region istypically a non-immunoglobulin protein and may be an enzyme, a toxin orprotein having known binding specificity. The two regions of this typeof chimeric antibody may be connected via a cleavable linker sequence.Immunoadhesins having the CDRs as hereinbefore described are alsocontemplated in the present invention.

The constant region is selected according to the functionality required.Normally an IgG1 will demonstrate lytic ability through binding tocomplement and/or will mediate ADCC (antibody dependent cellcytotoxicity). An IgG4 will be preferred if a non-cytotoxic blockingantibody is required. However, IgG4 antibodies can demonstrateinstability in production and therefore it may be more preferable tomodify the generally more stable IgG1. Suggested modifications aredescribed in EP0307434 preferred modifications include at positions 235and 237. The invention therefore provides a lytic or a non-lytic form ofan antibody according to the invention.

In one embodiment the antibody of the invention is a full length (i.e. atetramer comprising two heavy and two light chains) non-lytic IgG1antibody having the VH or VL sequences described supra. In anotherembodiment we provide a full length non-lytic IgG1 antibody having theVHs of SEQ ID NOs 11, 12, 16, 18 or 85, 86, 87 or 91; and VLs of SEQ IDNOs 20, 23 or 25.

In a further aspect, the invention provides polynucleotides encoding thedisclosed heavy or light chains or variable regions. For example theinvention provides polynucleotides encoding VH having the sequencecontained in SEQ ID NOs 45-52, 99-105 and VL regions having the sequencecontained in SEQ ID NOs 53-59.

“NOGO” refers to any NOGO polypeptide, including variant forms. Thisincludes, but is not limited to, NOGO-A having 1192 amino acid residues(GenBank accession no. AJ251383); NOGO-B, a splice variant which lacksresidues 186 to 1004 in the putative extracellular domain (GenBankaccession no. AJ251384) and a shorter splice variant, NOGO-C, which alsolacks residues 186 to 1004 and also has smaller, alternative aminoterminal domain (GenBank accession no. AJ251385) (Prinjha et al (2000)supra). All references to “NOGO” herein is understood to include any andall variant forms of NOGO such as NOGO-A and the splice variantsdescribed, unless a specific form is indicated.

“Neutralising” and grammatical variations thereof refers to inhibition,either total or partial, of NOGO function including its binding toneurones and inhibition of neurite growth.

“Altered antibody” refers to a protein encoded by an alteredimmunoglobulin coding region, which may be obtained by expression in aselected host cell. Such altered antibodies include engineeredantibodies (e.g., chimeric, reshaped, humanized or vectored antibodies)or antibody fragments lacking all or part of an immunoglobulin constantregion, e.g., Fv, Fab, or F(ab)₂ and the like.

“Altered immunoglobulin coding region” refers to a nucleic acid sequenceencoding altered antibody. When the altered antibody is a CDR-grafted orhumanized antibody, the sequences that encode the complementaritydetermining regions (CDRs) from a non-human immunoglobulin are insertedinto a first immunoglobulin partner comprising human variable frameworksequences. Optionally, the first immunoglobulin partner is operativelylinked to a second immunoglobulin partner.

The terms Fv, Fc, Fd, Fab, or F(ab)₂ are used with their standardmeanings (see, e.g., Harlow et al., Antibodies A Laboratory Manual, ColdSpring Harbor Laboratory, (1988)).

As used herein, an “engineered antibody” describes a type of alteredantibody, i.e., a full-length synthetic antibody (e.g., a chimeric,reshaped or humanized antibody as opposed to an antibody fragment) inwhich a portion of the light and/or heavy chain variable domains of aselected acceptor antibody are replaced by analogous parts from one ormore donor antibodies which have specificity for the selected epitope.For example, such molecules may include antibodies characterized by ahumanized heavy chain associated with an unmodified light chain (orchimeric light chain), or vice versa. Engineered antibodies may also becharacterized by alteration of the nucleic acid sequences encoding theacceptor antibody light and/or heavy variable domain framework regionsin order to retain donor antibody binding specificity. These antibodiescan comprise replacement of one or more CDRs (preferably all) from theacceptor antibody with CDRs from a donor antibody described herein.

A “chimeric antibody” refers to a type of engineered antibody whichcontains a naturally-occurring variable region (light chain and heavychains) derived from a donor antibody in association with light andheavy chain constant regions derived from an acceptor antibody.

A “humanized antibody” refers to a type of engineered antibody havingits CDRs derived from a non-human donor immunoglobulin, the remainingimmunoglobulin-derived parts of the molecule being derived from one (ormore) human immunoglobulin(s). In addition, framework support residuesmay be altered to preserve binding affinity (see, e.g., Queen et al.,Proc. Natl. Acad Sci USA, 86:10029-10032 (1989), Hodgson et al.,Bio/Technology, 9:421 (1991)). A suitable human acceptor antibody may beone selected from a conventional database, e.g., the KABAT® database,Los Alamos database, and Swiss Protein database, by homology to thenucleotide and amino acid sequences of the donor antibody. A humanantibody characterized by a homology to the framework regions of thedonor antibody (on an amino acid basis) may be suitable to provide aheavy chain constant region and/or a heavy chain variable frameworkregion for insertion of the donor CDRs. A suitable acceptor antibodycapable of donating light chain constant or variable framework regionsmay be selected in a similar manner. It should be noted that theacceptor antibody heavy and light chains are not required to originatefrom the same acceptor antibody. The prior art describes several ways ofproducing such humanised antibodies—see for example EP-A-0239400 andEP-A-054951

The term “donor antibody” refers to an antibody (monoclonal, and/orrecombinant) which contributes the amino acid sequences of its variableregions, CDRs, or other functional fragments or analogs thereof to afirst immunoglobulin partner, so as to provide the alteredimmunoglobulin coding region and resulting expressed altered antibodywith the antigenic specificity and neutralizing activity characteristicof the donor antibody.

The term “acceptor antibody” refers to an antibody (monoclonal and/orrecombinant) heterologous to the donor antibody, which contributes all(or any portion, but preferably all) of the amino acid sequencesencoding its heavy and/or light chain framework regions and/or its heavyand/or light chain constant regions to the first immunoglobulin partner.Preferably a human antibody is the acceptor antibody.

“CDRs” are defined as the complementarity determining region amino acidsequences of an antibody which are the hypervariable regions ofimmunoglobulin heavy and light chains. See, e.g., Kabat et al.,Sequences of Proteins of Immunological Interest, 4th Ed., U.S.Department of Health and Human Services, National Institutes of Health(1987). There are three heavy chain and three light chain CDRs (or CDRregions) in the variable portion of an immunoglobulin. Thus, “CDRs” asused herein refers to all three heavy chain CDRs, or all three lightchain CDRs (or both all heavy and all light chain CDRs, if appropriate).The structure and protein folding of the antibody may mean that otherresidues are considered part of the antigen binding region and would beunderstood to be so by a skilled person. See for example Chothia et al.,(1989) Conformations of immunoglobulin hypervariable regions; Nature342, p 877-883.

CDRs provide the majority of contact residues for the binding of theantibody to the antigen or epitope. CDRs of interest in this inventionare derived from donor antibody variable heavy and light chainsequences, and include analogs of the naturally occurring CDRs, whichanalogs also share or retain the same antigen binding specificity and/orneutralizing ability as the donor antibody from which they were derived.

A “functional fragment” is a partial heavy or light chain variablesequence (e.g., minor deletions at the amino or carboxy terminus of theimmunoglobulin variable region) which retains the same antigen bindingspecificity and the same or similar neutralizing ability as the antibodyfrom which the fragment was derived.

An “analog” is an amino acid sequence modified by at least one aminoacid, wherein said modification can be chemical or a substitution or arearrangement of a few amino acids (i.e., no more than 10), whichmodification permits the amino acid sequence to retain the biologicalcharacteristics, e.g., antigen specificity and high affinity, of theunmodified sequence.

Analogs may also arise as allelic variations. An “allelic variation ormodification” is an alteration in the nucleic acid sequence. Suchvariations or modifications may be due to degeneracy in the genetic codeor may be deliberately engineered to provide desired characteristics.These variations or modifications may or may not result in alterationsin any encoded amino acid sequence.

The present invention also includes the use of Fab fragments or F(ab′)₂fragments derived from mAbs of the present invention directed againstNOGO. A Fab fragment contains the entire light chain and amino terminalportion of the heavy chain; and an F(ab′)₂ fragment is the fragmentformed by two Fab fragments bound by disulfide bonds. Fab fragments andF(ab′)₂ fragments can be obtained by conventional means, e.g., cleavageof mAb with the appropriate proteolytic enzymes, papain and/or pepsin,or by recombinant methods. The Fab and F(ab′)₂ fragments are usefulthemselves as therapeutic or prophylactic, and as donors of sequencesincluding the variable regions and CDR sequences useful in the formationof recombinant or humanized antibodies as described herein.

Altered immunoglobulin molecules can encode altered antibodies whichinclude engineered antibodies such as chimeric antibodies and humanizedantibodies. A desired altered immunoglobulin coding region containsCDR-encoding regions that encode peptides having the antigen specificityof an anti-NOGO antibody, preferably a high affinity antibody, insertedinto a first immunoglobulin partner (a human framework or humanimmunoglobulin variable region).

Preferably, the first immunoglobulin partner is operatively linked to asecond immunoglobulin partner. The second immunoglobulin partner isdefined above, and may include a sequence encoding a second antibodyregion of interest, for example an Fc region. Second immunoglobulinpartners may also include sequences encoding another immunoglobulin towhich the light or heavy chain constant region is fused in frame or bymeans of a linker sequence. Engineered antibodies directed againstfunctional fragments or analogs of NOGO may be designed to elicitenhanced binding.

The second immunoglobulin partner may also be associated with effectoragents as defined above, including non-protein carrier molecules, towhich the second immunoglobulin partner may be operatively linked byconventional means.

Fusion or linkage between the second immunoglobulin partners, e.g.,antibody sequences, and the effector agent may be by any suitable means,e.g., by conventional covalent or ionic bonds, protein fusions, orhetero-bifunctional cross-linkers, e.g., carbodiimide, glutaraldehyde,and the like. Such techniques are known in the art and readily describedin conventional chemistry and biochemistry texts.

Additionally, conventional linker sequences which simply provide for adesired amount of space between the second immunoglobulin partner andthe effector agent may also be constructed into the alteredimmunoglobulin coding region. The design of such linkers is well knownto those of skill in the art. In further aspects of the invention weprovide diabodies (bivalent or bispecific), triabodies, tetrabodies andother multivalent scFV protein species having one or more CDRs asdescribed supra that bind to and neutralise NOGO function.

In still a further embodiment, the antibody of the invention may haveattached to it an additional agent. For example, the procedure ofrecombinant DNA technology may be used to produce an engineered antibodyof the invention in which the Fc fragment or CH2-CH3 domain of a fulllength antibody molecule has been replaced by an enzyme or otherdetectable molecule (i.e., a polypeptide effector or reporter molecule).

The second immunoglobulin partner may also be operatively linked to anon-immunoglobulin peptide, protein or fragment thereof heterologous tothe CDR-containing sequence having the antigen specificity of anti-NOGOantibody. The resulting protein may exhibit both anti-NOGO antigenspecificity and characteristics of the non-immunoglobulin uponexpression. That fusion partner characteristic may be, e.g., afunctional characteristic such as another binding or receptor domain, ora therapeutic characteristic if the fusion partner is itself atherapeutic protein, or additional antigenic characteristics.

Another desirable protein of this invention may comprise a full lengthantibody molecule, having full length heavy and light chains, or anydiscrete fragment thereof, such as the Fab or F(ab′)₂ fragments, a heavychain dimer, or any minimal recombinant fragments thereof such as an Fvor a single-chain antibody (SCA) or any other molecule with the samespecificity as the selected donor mAb. Such protein may be used in theform of an altered antibody, or may be used in its unfused form.

Whenever the second immunoglobulin partner is derived from an antibodydifferent from the donor antibody, e.g. any isotype or class ofimmunoglobulin framework or constant regions, an engineered antibodyresults. Engineered antibodies can comprise immunoglobulin (Ig) constantregions and variable framework regions from one source, e.g., theacceptor antibody, and one or more (preferably all) CDRs from the donorantibody. In addition, alterations, e.g., deletions, substitutions, oradditions, of the acceptor mAb light and/or heavy variable domainframework region at the nucleic acid or amino acid levels, or the donorCDR regions may be made in order to retain donor antibody antigenbinding specificity.

Such engineered antibodies are designed to employ one (or both) of thevariable heavy and/or light chains of the anti-NOGO mAb or one or moreof the heavy or light chain CDRs. The engineered antibodies may beneutralising, as above defined.

In addition, the constant region may be altered to enhance or decreaseselective properties of the molecules of the instant invention. Forexample, dimerization, binding to Fc receptors, or the ability to bindand activate complement (see, e.g., Angal et al., Mol. Immunol,30:105-108 (1993), Xu et al., J. Biol. Chem., 269:3469-3474 (1994),Winter et al., EP 307,434-B).

The antibodies of the present invention may be produced by making aconventional expression vector or recombinant plasmid by placing thesecoding sequences for the antibody in operative association withconventional regulatory control sequences capable of controlling thereplication and expression in, and/or secretion from, a host cell.Regulatory sequences include promoter sequences, e.g., CMV promoter, andsignal sequences, which can be derived from other known antibodies.Similarly, a second expression vector can be produced having a DNAsequence which encodes a complementary antibody light or heavy chain.Preferably this second expression vector is identical to the firstexcept insofar as the coding sequences and selectable markers areconcerned, so to ensure as far as possible that each polypeptide chainis functionally expressed. Alternatively, the heavy and light chaincoding sequences for the altered antibody may reside on a single vector.

A selected host cell is co-transfected by conventional techniques withboth the first and second vectors (or simply transfected by a singlevector) to create the transfected host cell of the invention comprisingboth the recombinant or synthetic light and heavy chains. Thetransfected cell is then cultured by conventional techniques to producethe engineered antibody of the invention. The antibody which includesthe association of both the recombinant heavy chain and/or light chainis screened from culture by appropriate assay, such as ELISA or RIA.Similar conventional techniques may be employed to construct otheraltered antibodies and molecules.

Suitable vectors for the cloning and subcloning steps employed in themethods and construction of the compositions of this invention may beselected by one of skill in the art. For example, the conventional pUCseries of cloning vectors may be used. One vector, pUC19, iscommercially available from supply houses, such as Amersham(Buckinghamshire, United Kingdom) or Pharmacia (Uppsala, Sweden).Additionally, any vector which is capable of replicating readily, has anabundance of cloning sites and selectable genes (e.g., antibioticresistance), and is easily manipulated may be used for cloning. Thus,the selection of the cloning vector is not a limiting factor in thisinvention.

Similarly, the vectors employed for expression of the antibodies may beselected by one of skill in the art from any conventional vector. Thevectors also contain selected regulatory sequences (such as CMV or RSVpromoters) which direct the replication and expression of heterologousDNA sequences in selected host cells. These vectors contain the abovedescribed DNA sequences which code for the antibody or alteredimmunoglobulin coding region. In addition, the vectors may incorporatethe selected immunoglobulin sequences modified by the insertion ofdesirable restriction sites for ready manipulation.

The expression vectors may also be characterized by genes suitable foramplifying expression of the heterologous DNA sequences, e.g., themammalian dihydrofolate reductase gene (DHFR). Other preferable vectorsequences include a poly A signal sequence, such as from bovine growthhormone (BGH) and the betaglobin promoter sequence (betaglopro). Theexpression vectors useful herein may be synthesized by techniques wellknown to those skilled in this art.

The components of such vectors, e.g. replicons, selection genes,enhancers, promoters, signal sequences and the like, may be obtainedfrom commercial or natural sources or synthesized by known proceduresfor use in directing the expression and/or secretion of the product ofthe recombinant DNA in a selected host. Other appropriate expressionvectors of which numerous types are known in the art for mammalian,bacterial, insect, yeast, and fungal expression may also be selected forthis purpose.

The present invention also encompasses a cell line transfected with arecombinant plasmid containing the coding sequences of the antibodies oraltered immunoglobulin molecules thereof. Host cells useful for thecloning and other manipulations of these cloning vectors are alsoconventional. However, most desirably, cells from various strains of E.coli are used for replication of the cloning vectors and other steps inthe construction of altered antibodies of this invention.

Suitable host cells or cell lines for the expression of the antibody ofthe invention are preferably mammalian cells such as NS0, Sp2/0, CHO(e.g. DG44), COS, a fibroblast cell (e.g., 3T3), and myeloma cells, andmore preferably a CHO or a myeloma cell. Human cells may be used, thusenabling the molecule to be modified with human glycosylation patterns.Alternatively, other eukaryotic cell lines may be employed. Theselection of suitable mammalian host cells and methods fortransformation, culture, amplification, screening and product productionand purification are known in the art. See, e.g., Sambrook et al., citedabove.

Bacterial cells may prove useful as host cells suitable for theexpression of the recombinant Fabs of the present invention (see, e.g.,Plückthun, A., Immunol. Rev., 130:151-188 (1992)). However, due to thetendency of proteins expressed in bacterial cells to be in an unfoldedor improperly folded form or in a non-glycosylated form, any recombinantFab produced in a bacterial cell would have to be screened for retentionof antigen binding ability. If the molecule expressed by the bacterialcell was produced in a properly folded form, that bacterial cell wouldbe a desirable host. For example, various strains of E. coli used forexpression are well-known as host cells in the field of biotechnology.Various strains of B. subtilis, Streptomyces, other bacilli and the likemay also be employed in this method.

Where desired, strains of yeast cells known to those skilled in the artare also available as host cells, as well as insect cells, e.g.Drosophila and Lepidoptera and viral expression systems. See, e.g.Miller et al., Genetic Engineering, 8:277-298, Plenum Press (1986) andreferences cited therein.

The general methods by which the vectors may be constructed, thetransfection methods required to produce the host cells of theinvention, and culture methods necessary to produce the antibody of theinvention from such host cell are all conventional techniques.Typically, the culture method of the present invention is a serum-freeculture method, usually by culturing cells serum-free in suspension.Likewise, once produced, the antibodies of the invention may be purifiedfrom the cell culture contents according to standard procedures of theart, including ammonium sulfate precipitation, affinity columns, columnchromatography, gel electrophoresis and the like. Such techniques arewithin the skill of the art and do not limit this invention. Forexample, preparation of altered antibodies are described in WO 99/58679and WO 96/16990.

Yet another method of expression of the antibodies may utilizeexpression in a transgenic animal, such as described in U.S. Pat. No.4,873,316. This relates to an expression system using the animal'scasein promoter which when transgenically incorporated into a mammalpermits the female to produce the desired recombinant protein in itsmilk.

In a further aspect of the invention there is provided a method ofproducing an antibody of the invention which method comprises the stepof culturing a host cell transformed or transfected with a vectorencoding the light and/or heavy chain of the antibody of the inventionand recovering the antibody thereby produced.

In accordance with the present invention there is provided a method ofproducing an anti-NOGO antibody which specifically binds to andneutralises the activity of human NOGO-A which method comprises thesteps of;

-   -   (a) providing a first vector encoding a heavy chain of the        antibody;    -   (b) providing a second vector encoding the light chain of the        antibody;    -   (c) transforming a mammalian host cell (e.g. CHO) with said        first and second vectors;    -   (d) culturing the host cell of step (c) under conditions        conducive to the secretion of the antibody from said host cell        into said culture media;    -   (e) recovering the secreted antibody of step (d).

Once expressed by the desired method, the antibody is then examined forin vitro activity by use of an appropriate assay. Presently conventionalELISA assay formats are employed to assess qualitative and quantitativebinding of the antibody to NOGO. Additionally, other in vitro assays mayalso be used to verify neutralizing efficacy prior to subsequent humanclinical studies performed to evaluate the persistence of the antibodyin the body despite the usual clearance mechanisms.

The therapeutic agents of this invention may be administered as aprophylactic or following the stroke event/on-set of clinical symptoms,or as otherwise needed. The dose and duration of treatment relates tothe relative duration of the molecules of the present invention in thehuman circulation, and can be adjusted by one of skill in the artdepending upon the condition being treated and the general health of thepatient. It is envisaged that repeated dosing (e.g. once a week or onceevery two weeks) over an extended time period (e.g. four to six months)maybe required to achieve maximal therapeutic efficacy.

The mode of administration of the therapeutic agent of the invention maybe any suitable route which delivers the agent to the host. Theantagonists and antibodies, and pharmaceutical compositions of theinvention are particularly useful for parenteral administration, i.e.,subcutaneously, intrathecally, intraperitoneally, intramuscularly,intravenously, or intranasally, of which intravenously is particularlypreferred.

Therapeutic agents of the invention may be prepared as pharmaceuticalcompositions containing an effective amount of the antagonist orantibody of the invention as an active ingredient in a pharmaceuticallyacceptable carrier. In the prophylactic agent of the invention, anaqueous suspension or solution containing the engineered antibody,preferably buffered at physiological pH, in a form ready for injectionis preferred. The compositions for parenteral administration willcommonly comprise a solution of the antagonist or antibody of theinvention or a cocktail thereof dissolved in a pharmaceuticallyacceptable carrier, preferably an aqueous carrier. A variety of aqueouscarriers may be employed, e.g., 0.9% saline, 0.3% glycine, and the like.These solutions are sterile and generally free of particulate matter.These solutions may be sterilized by conventional, well knownsterilization techniques (e.g., filtration). The compositions maycontain pharmaceutically acceptable auxiliary substances as required toapproximate physiological conditions such as pH adjusting and bufferingagents, etc. The concentration of the antagonist or antibody of theinvention in such pharmaceutical formulation can vary widely, i.e., fromless than about 0.5%, usually at or at least about 1′)/0 to as much as15 or 20% by weight and will be selected primarily based on fluidvolumes, viscosities, etc., according to the particular mode ofadministration selected.

Thus, a pharmaceutical composition of the invention for intramuscularinjection could be prepared to contain 1 mL sterile buffered water, andbetween about 1 ng to about 100 mg, e.g. about 50 ng to about 30 mg ormore preferably, about 5 mg to about 25 mg, of an antagonist or antibodyof the invention. Similarly, a pharmaceutical composition of theinvention for intravenous infusion could be made up to contain about 250ml of sterile Ringer's solution, and about 1 to about 30 and preferably5 mg to about 25 mg of an engineered antibody of the invention. Actualmethods for preparing parenterally administrable compositions are wellknown or will be apparent to those skilled in the art and are describedin more detail in, for example, Remington's Pharmaceutical Science, 15thed., Mack Publishing Company, Easton, Pa. For the preparation ofintravenously administrable antibody formulations of the invention seeLasmar U and Parkins D “The formulation of Biopharmaceutical products”,Pharma. Sci. Tech. today, page 129-137, Vol. 3 (3 Apr. 2000), Wang, W“Instability, stabilisation and formulation of liquid proteinpharmaceuticals”, Int. J. Pharm 185 (1999) 129-188, Stability of ProteinPharmaceuticals Part A and Bed Ahern T. J., Manning M. C., New York,N.Y.: Plenum Press (1992), Akers, M. J. “Excipient-Drug interactions inParenteral Formulations”, J. Pharm Sci 91 (2002) 2283-2300, Imamura, Ket al “Effects of types of sugar on stabilization of Protein in thedried state”, J Pharm Sci 92 (2003) 266-274, Izutsu, Kkojima, S.“Excipient crystallinity and its protein-structure-stabilizing effectduring freeze-drying”, J. Pharm. Pharmacol, 54 (2002) 1033-1039,Johnson, R, “Mannitol-sucrose mixtures-versatile formulations forprotein lyophilization”, J. Pharm. Sci, 91 (2002) 914-922.

Ha, E Wang W, Wang Y. j. “Peroxide formation in polysorbate 80 andprotein stability”, J. Pharm Sci, 91, 2252-2264, (2002) the entirecontents of which are incorporated herein by reference and to which thereader is specifically referred.

It is preferred that the therapeutic agent of the invention, when in apharmaceutical preparation, be present in unit dose forms. Theappropriate therapeutically effective dose can be determined readily bythose of skill in the art. To effectively treat stroke and otherneurological diseases in a human, one dose of up to 700 mg per 70 kgbody weight of an antagonist or antibody of this invention should beadministered parenterally, preferably i.v. or i.m. (intramuscularly).Such dose may, if necessary, be repeated at appropriate time intervalsselected as appropriate by a physician. As disclosed in the examples,the present inventors have been able to demonstrate a positive effect onfunctional recovery in the rat model therein when antibodies of theinvention were administered intravenously.

The antibodies described herein can be lyophilized for storage andreconstituted in a suitable carrier prior to use. This technique hasbeen shown to be effective with conventional immunoglobulins andart-known lyophilization and reconstitution techniques can be employed.

Antibodies of the invention may also be used in combination (i.e.simultaneously, sequentially or separately) with a neurotrophic factorsuch as nerve growth factor (NGF), for example brain derivedneurotrophic factor (BDNF), anti-inflammatory agents such ascorticosteroids, and/or tPA. Combinations of a NOGO antibody of theinvention and e.g. tPA maybe assessed in the MCAO model set forth in theexamples below.

In another aspect, the invention provides a pharmaceutical compositioncomprising anti-NOGO antibody of the present invention or a functionalfragment thereof and a pharmaceutically acceptable carrier for treatmentor prophylaxis of stroke and other neurological diseases.

In a yet further aspect, the invention provides a pharmaceuticalcomposition comprising the anti-NOGO antibody of the present inventionor a functional fragment thereof and a pharmaceutically acceptablecarrier for inhibiting neurodegeneration and/or promoting functionalrecovery in a human patient suffering, or at risk of developing, astroke or other neurological disease.

The invention further provides a method of treatment or prophylaxis ofstroke (particularly ischemic stroke) and other neurologicaldiseases/disorders, in particular Alzheimer's disease, in a human whichcomprises administering to said human in need thereof an effectiveamount of an anti-NOGO antibody or a functional fragment thereof.Antibodies of the invention may be used in methods of treatment to slowor halt the progression and/or onset of Alzheimer's disease in additionto (or as an alternative to) treating established disease in a humanpatient.

Further the invention provides the use of an anti-NOGO antibody, or afunctional fragment thereof, in the preparation of a medicament fortreatment or prophylaxis of stroke and other neurologicaldiseases/disorders, in particular Alzheimer's disease.

The invention also provides a method of inhibiting neurodegenerationand/or promoting functional recovery in a human patient suffering, or atrisk of developing, a stroke or other neurological disease/disorder, inparticular Alzheimer's disease, which comprises administering to saidhuman in need thereof an effective amount of an anti-NOGO antibody or afunctional fragment thereof.

In addition the invention provides the use of an anti-NOGO antibody or afunctional fragment thereof in the preparation of a medicament forinhibiting neurodegeneration and/or promoting functional recovery in ahuman patient afflicted with, or at risk of developing, a stroke andother neurological disease/disorder, in particular Alzheimer's disease.

The invention further provides a method of treating or prophylaxis ofstroke or other neurological disease/disorder, in particular Alzheimer'sdisease, in a human comprising the step of parenteral administration ofa therapeutically effective amount of an anti-NOGO antibody. Preferablythe anti-NOGO antibody is administered intravenously.

Neurological diseases or disorders as used hereinabove includes, but isnot limited to traumatic brain injury, spinal cord injury,fronto-temporal dementias (tauopathies), peripheral neuropathy,Parkinson's disease, Huntington's disease, multiple sclerosis and inparticular Alzheimer's disease.

The invention also provides a method of promoting axonal sproutingcomprising the step of contacting a human axon with an anti-NOGOantibody. This method may be performed in-vitro or in-vivo, preferablythe method is performed in-vivo.

In a further aspect therefore there is provided the use of an anti-NOGOantibody or functional fragment thereof of the invention comprisingCDR's of table 1 and 2; CDR's of Table 3 and 4; or CDR's of table 5 and6 in intravenously administrable form in the manufacture of a medicamentfor the treatment of stroke (particularly ischemic stroke), braininjury, spinal cord injury, fronto-temporal dementias (tauopathies),peripheral neuropathy, Parkinson's disease, Huntington's disease,multiple sclerosis and in particular Alzheimer's disease in a humanpatient.

In a further aspect therefore there is provided a method of treatingstroke (particularly ischemic stroke), brain injury, spinal cord injury,fronto-temporal dementias (tauopathies), peripheral neuropathy,Parkinson's disease, Huntington's disease, multiple sclerosis and inparticular Alzheimer's disease in a human patient which method comprisesthe intravenous administration of a therapeutically effective amount ofan anti-NOGO antibody of the invention.

In a further aspect of the present invention there is provided a methodof promoting axon sprouting of neurons within the central nervous systemof a human subject (e.g. patient) which method comprises administering(e.g. intravenously administering) a therapeutically effective amount ofan anti-NOGO antibody (e.g. an anti-NOGO antibody comprising CDRs as setforth herein).

In a further aspect of the present invention there is provided the useof an anti-NOGO antibody (e.g. an anti-NOGO antibody comprising the CDRsset forth herein) in the manufacture of an intravenously administrablemedicament for the treatment of stroke (particularly ischemic stroke),brain injury, spinal cord injury, fronto-temporal dementias(tauopathies), peripheral neuropathy, Parkinson's disease, Huntington'sdisease, multiple sclerosis and in particular Alzheimer's disease in ahuman patient.

In a further aspect of the invention there is provided a method ofregenerating axon processes in neurons of the central nervous system ina human patient afflicted with (or susceptible to) stroke (particularlyischemic stroke), brain injury, spinal cord injury, fronto-temporaldementias (tauopathies), peripheral neuropathy, Parkinson's disease,Huntington's disease, multiple sclerosis and in particular Alzheimer'sdisease which method comprises the step of administering (e.g.intravenously) a therapeutically effective amount of an anti-NOGOantibody (e.g. an anti-NOGO antibody having the CDRs set forth herein).

In a further aspect of the invention there is provided the use of ananti-NOGO antibody (e.g. an anti-NOGO antibody having the CDRs set forthherein) in the manufacture of an intravenously administrablepharmaceutical composition for regenerating axon processes in neurons ofthe central nervous system in a human patient afflicted with (orsusceptible to) stroke (particularly ischemic stroke), brain injury,spinal cord injury, fronto-temporal dementias (tauopathies), peripheralneuropathy, Parkinson's disease, Huntington's disease, multiplesclerosis and in particular Alzheimer's disease.

In a further aspect of the invention there is provided a method ofmodulating the production of an amyloidogenic peptide comprisingcontacting a cell which is expressing the precursor from which theamyloidogenic peptide is derived and a NOGO polypeptide (e.g. humanNOGO-A) with an anti-NOGO antibody (e.g. an anti-NOGO antibodycomprising the CDRs set forth herein, particularly 2A10 and fully humanor humanised versions thereof). In typical embodiments, the precursor isAPP. In further typical embodiments the amyloidogenic peptide is Aβ,most preferably Aβ40, Aβ42 or a combination of both.

As used herein, the term “functional recovery” refers to a motor and/orsensory and/or behavioural improvement in a subject following e.g. anischemic event or injury or on-set of clinical symptoms. Functionalrecovery in humans may be evaluated by instruments designed to measureelemental neurological functions such as motor strength, sensation andcoordination, cognitive functions such as memory, language and theability to follow directions, and functional capacities such as basicactivities of daily living or instrumental activities. Recovery ofelemental neurological function can be measured with instruments such asthe NIH Stroke Scale (NIHSS), recovery of cognitive function can bemeasured with neuropsychological tests such as Boston Naming Test,Trail-making Tests, and California Verbal Learning Test, and activitiesof daily living may be measured with instruments such as the ADCS/ADL(Alzheimer's Disease Clinical Studies/Activities of Daily Living) scaleor the Bristol Activities of Daily Living Scale, all tests and scalesknown in the art.

Example 1, Construction and Expression of Humanised Anti-NOGO Antibodies

Murine and Humanised V_(H) and V_(L) constructs were prepared de novo bybuild up of overlapping oligonucleotides including restriction sites forcloning into Rld and Rln mammalian expression vectors as well as a humansignal sequence. Hind III and Spe I restriction sites were introduced toframe the V_(H) domain containing the CAMPATH-1H signal sequence forcloning into Rld containing the human γ1 mutated constant region. HindIII and BsiWI restriction sites were introduced to frame the V_(L)domain containing the CAMPATH-1H signal sequence for cloning into Rlncontaining the human kappa constant region.

CAMPATH-1H signal sequence: (SEQ. I.D. NO: 82) MGWSCIILFLVATATGVHSIn parallel a chimeric version of 11C7 was generated (see WO04/052932).The variable heavy domain sequence (derived from WO04/052932 Seq ID 43)and variable light domain sequence (derived from WO04/052932 Seq ID 44)were prepared de novo by build up of overlapping oligonucleotides. HindIII and SpeI restriction sites were introduced to frame the V_(H) domainfor cloning into Rld containing the human γ1 mutated constant region.HindIII and BsiWI restriction sites were introduced to frame the V_(L)domain for cloning into Rln containing the human kappa constant region.

Example 2, Antibody Expression in CHO Cells

Rld and Rln plasmids encoding the heavy and light chains respectivelywere transiently co-transfected into CHO cells and expressed at smallscale or large scale to produce antibody. Alternatively the sameplasmids were co-transfected into CHO cells by electroporation and astable polyclonal population of cells expressing the appropriateantibody were selected using a nucleoside-free media. Recombinantantibody was recovered and purified by affinity chromatography onProtein A sepharose.

Example 3, Humanised Anti-NOGO Antibody Binds to NOGO

GST-human NOGO-A56 (SEQ ID: 76) at 1 μg/ml in PBS was coated onto NuncImmunosorp plates (100 μl per well) at 4° C. overnight. Wells wererinsed once with TBS+0.05% Tween (TBST) then incubated with 2% BSA inTBST to block non-specific binding sites at room temperature for 1 hour.Antibodies were diluted in TBST+2% BSA to 10 μg/ml and 1/2 dilutionsmade from this. Antibodies were added to wells in duplicate andincubated at room temperature for 1 hour. Wells were washed three timeswith TBST then incubated with anti-human kappa peroxidase conjugate(1:2000) for 1 hour. The wells were washed three times with TBST andthen incubated with 100 μl OPD peroxidase substrate (Sigma) per well for10 minutes. The colour reaction was stopped by the addition of 25 μlconcentrated H₂SO₄. Optical density at 490 nm was measured using a platereader. Background values read from wells with no antibody weresubtracted.

FIGS. 1-5 illustrate the dose-dependent binding of humanised antibodiesin comparison with the chimera (termed HcLc which is the chimera of 2A10(comprising the 2A10 murine VH (SEQ ID NO. 7) and VL (SEQ ID NO. 8) andhuman IgG constant regions)) to human NOGO-A56 (see Example 6 fordetails) in an ELISA assay. The Y-axis shows the measured opticaldensity (OD) at 490 nm, a quantitative measure of antibody captured inthe wells. The X-axis shows the concentration of antibody used (mcg/ml)per well at each data point.

The antibody material used in FIGS. 1, 3, 4 and 5 was generated fromsmall scale transient transfections. Human IgG levels in the supernatantare quantified by ELISA (Example 4). For FIG. 2, the material used ispurified antibody generated by either the polyclonal expression systemor large scale transient transfections. In these cases, IgG levels werequantified by ELISA and optical density.

In another experiment, antibody material was generated from small scaletransient transfections (in triplicate) for the following humanisedantibodies: H16L16, H17L16, H18L16, H16L18 and the chimeric antibodyHcLc. The results from this experiment are consistent with the datapresented in FIGS. 1-5 with the exception of H17L16 which performed lesswell than shown in FIG. 1A and FIG. 2. Whilst this observation cannot beexplained it should be noted that the conclusions are contradicted byanother experiment with supernatant material (see FIG. 1A) and anexperiment using purified H17L16 material (FIG. 2), both experimentsindicated that H17L16 shows comparable binding to the other optimisedvariants.

Example 4, Antibody Quantification Protocol

Nunc Immunosorp plates were coated with capture antibody H19 (goatanti-human IgG chain, Sigma #13382) at 2 μg/ml in Bicarbonate buffer(Sigma #C3041) and incubated overnight at 4° C. The plates were washedtwice with TBS containing 0.05% Tween20 (TBST) and blocked with 200 μlTBST containing 2% BSA (block buffer) for 1 hr at room temperature. Theplates were washed twice with TBST. Tissue culture supernatantscontaining antibody were titrated across the plate in 2-fold dilutionsteps into block buffer and incubated at room temperature for 1 hr. Theplates were washed three times with TBST. HRP conjugated antibody H23(goat anti-human kappa chain, Sigma #A7164) was diluted 1:2000 in TBSTand 100 μl added to each well. The plates were incubated at roomtemperature for 1 hr. The plates were washed three times with TBST anddeveloped with 100 μl of Fast-OPD substrate (Sigma #P9187). Colour wasallowed to develop for 5-10 mins after which time the ELISA was stoppedwith 25 μl 3M H₂SO₄. The absorbance at 490 nM was read plate andantibody concentration determined by reference to a standard curve.

Example 5, Antibody Competition ELISA Protocol

GST-human NOGO-A56 (SEQ ID: 76) at 0.1-1.0 μg/ml in PBS was coated ontoNunc Immunosorp plates (100 μl per well) at 4° C. overnight or at 37° C.for 1 hour. Wells were rinsed three times with PBS then incubated with1% BSA in PBS (block buffer) to block non-specific binding sites at roomtemperature for 2 hours. In parallel, a 50:50 mix of antibodies wasmade. Murine antibody 2A10 was added to a final concentration of either0.5 or 1.0 mcg/ml in block buffer. Chimeric antibodies (mouse variableregions cloned onto a human IgG1Fc mutated constant region) were addedto a final concentration of 0-25 mcg/ml in block buffer. The blockbuffer was removed from the plates and 100 μl of the 50:50 mix ofantibodies was added for 1 hour at room temperature. Wells were washedthree times with PBS then incubated with 100 μl of rabbit polyclonalanti-mouse immunoglobulins peroxidase conjugate (diluted 1:2000 in blockbuffer, DakoCytomation #P0260) for 1 hour at room temperature. The wellswere washed three times with PBS and then incubated with 100 μl OPDperoxidase substrate (Sigma #P9187) or TMB substrate (Sigma #T8665) perwell for 10-30 minutes. The colour reaction was stopped by the additionof 25 μl concentrated H₂SO₄. Optical density at 490 nm (OPD) or 450 nm(TMB) was measured using a plate reader.

In the first experiment (FIG. 7A), plates were coated with GST-humanNOGO-A56 at 0.5 μg/ml in PBS overnight at 4° C. and the plates weredeveloped with TMB substrate. In this experiment, the murine antibody2A10 was assessed in combination with HcLc (the chimeric form of 2A10),11C7, an isotype control chimeric antibody and a blank control. In thesecond experiment (FIG. 7B), plates were coated with GST-human NOGO-A56at 0.5 μg/ml in PBS at 37° C. for 1 hour and the plates were developedwith OPD substrate. In this experiment, the murine antibody 2A10 wasassessed in combination with HcLc, 11C7, an isotype control and H16L18.

Example 6, Production of NOGO-A Fragment (NOGO-A56, SEQ. I.D. NO:76)

A cDNA sequence encoding amino acids 586-785(MQESLYPAAQLCPSFEESEATPSPVLPDIVMEAPLNSAVPSAGASVIQPSSSPLEASSVNYESIKHEPENPPPYEEAMSVSLKKVSGIKEEIKEPENINAALQETEAPYISIACDLIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSDDSIPDVPQKQDETVMLVKESLTETSFESMIEYENKE—SEQ. I.D. NO:76) of human NOGO-A was clonedinto the BamHI-XhoI sites of pGEX-6P1 to generate a GST-tagged fusionprotein designated NOGO-A56. Plasmid was expressed in BL21 cells in 2×TYmedium with 100 μg/ml ampicillin following induction with IPTG to 0.5 mMat 37 C for 3 hours. Cell pellets were lysed by sonication and thefusion protein purified using Glutathione-sepharose (Amersham Pharmacia)following manufacturers instructions. Purified protein was eluted usingreduced glutathione and extensively dialysed against PBS, quantitatedusing BSA standards and a BioRad coomassie based protein assay and thenstored in aliquots at −80 C.

Example 7, BiaCore Analysis of Humanised Anti NOGO Monoclonal Antibodies

The binding kinetics of the anti-NOGO monoclonal antibody (mAb) torecombinantly expressed human NOGO-A (GST-human NOGO-A56) was analysedusing the Biacore3000 biosensor. The hNOGO-A chip was prepared asfollows:

Method

hNOGO (GST-human NOGO-A56) was immobilised to a CM5 chip by primaryamine coupling using the Biacore Wizard program designed for targetedimmobilisation levels. The CM5 sensor surface was activated by passing asolution of 50 mM N-hydroxy-succinimide (NHS) and 200 mMN-ethyl-N′-dimethylaminopropyl carbonide (EDC). Then hNOGO in sodiumacetate buffer, pH5.0 or pH 4.5, was passed over the chip andimmobilised. After immobilisation was complete any still activatedesters were blocked by an injection of 1M ethanolamine hydrochloride,pH8.5.

The anti-NOGO mAbs were diluted down in HBS-EP (10 mM HEPES, pH 7.4, 150mM NaCl, 3 mM EDTA, and 0.005% P-20 surfactant) and binding studies werecarried out at range of defined antibody concentrations. All runs werereferenced against a blanked sensor surface (one that had been activatedand blocked as described earlier but had no addition of ligand).Analysis of binding was carried out using the BIAevaluation kineticanalysis software version 4.1. Biacore analysis of other antibodies ofthe invention essentially followed the same protocol as describedherein.

TABLE 8 Results Mean (+/−standard deviation) of four separateexperiments Antibody ka (1/Ms) kd (1/s) KD (nM) HcLc 2.70e6 (2.7e5)3.78e−3 (7.0e−4) 1.41 (0.3) H6L13 1.82e6 (3.5e5) 1.37e−2 (2.0e−3) 7.68(1.2) H16L16 4.37e6 (4.5e5) 5.54e−3 (1.4e−3) 1.26 (0.2) H16L18 4.18e6(4.1e5) 5.52e−3 (9.0e−4) 1.33 (0.2) H17L16 3.38e6 (1.3e5) 6.10e−3(1.3e−3) 1.82 (0.4) H18L16 3.64e6 (3.5e5) 5.86e−3 (9.0e−4) 1.62 (0.3)H1L11 1.73e6 (1.7e5) 3.14e−2 (4.2e−3) 18.6 (3.7)

TABLE 9 Results Results shown are from a single experiment with theexception of HcLc and H6Lc where the values shown are the mean(+/−standard deviation) of two separate experiments Antibody (number ofindependent runs) ka (1/Ms) kd (1/s) KD (nM) HcLc (2) 3.52e6 (2.8e5)3.46e−3 (7.9e−4) 0.995 (0.3) HcL11 3.78e6 1.34e−2 3.55 H6Lc (2) 2.17e6(3.8e5) 2.84e−3 (1.5e−3)  2.21 (0.3) HcL13  4.8e6 9.38e−3 1.98 H6L132.95e6 2.33e−2 7.9 H6L6  1.2e6 2.54e−2 21.4 H10L13 1.64e6 2.12e−2 12.95H700L11 1.19e6 2.33e−2 19.45 H5L6  1.5e6  4.7e−2 30.3 11C7 1.44e68.25e−5 0.057

TABLE 10 Results Results shown are from a single experiment Antibody ka(1/Ms) kd (1/s) KD (nM) H6L13 1.77e6  1.3e−2 7.3 H16L16 5.47e6 5.69e−31.0 H14L16 2.05e6 7.39e−3 3.6 H14L18 2.41e6 8.69e−3 3.6 H1L11 1.33e62.84e−2 21.3 HcLc 2.62e6 4.12e−3 1.6

Example 8, BiaCore Analysis of Humanised Anti NOGO Monoclonal AntibodiesUsing Off-Rate Ranking

The hNOGO chip was prepared as for kinetic analysis. Cell supernatantswhere taken directly from transient transfections of CHO-K1 cells. Thesewere passed directly over the sensor surface and the interactionmeasured. A mock transfected cell supernatant was used for doublereferencing to remove any artefacts due to the tissue culture media. Allruns were referenced against a blanked sensor surface (one that had beenactivated and blocked as described earlier but had no addition ofligand). Analysis of binding was carried out using the BIAevaluationkinetic analysis software version 4.1.

TABLE 11 Results Results shown are from a single experiment with theexception of H6L13, H16L16, H16L18, H1L11, HcLc and H18L16 where thevalues shown are the mean (+/−standard deviation) of two or threeseparate experiments Antibody (number of independent runs) kd (1/s)H14L13 1.38e−2 H15L13 9.65e−3 H16L13 9.07e−3 H17L13 9.31e−3 H18L139.07e−3 H6L13 (x3) 1.73e−2 (4.8e−3) H16L16 (x3) 6.64e−3 (9.2e−4) H16L18(x3) 6.09e−3 (7.4e−4) H1L11 (x3) 4.03e−2 (1.8e−2) HcLc (x2) 3.76e−3(7.1e−4) H15L16 6.04e−3 H14L16  8.9e−3 H18L16 (x2) 6.87e−3 (7.5e−4)H14L18 8.35e−3 H15L18 5.94e−3 H18L18  5.8e−3 H6L17 1.58e−2 H6L18 1.06e−2H6L14 4.57e−2 H6L15 2.11e−2 H6L16 1.14e−2

Example 9, FACS Analysis of Humanised Anti NOGO Monoclonal Antibodies

IMR32 human neuroblastoma cells were re-suspended in FACS stainingbuffer (PBS+4% heat inactivated FCS) at a density of 10⁶ cells per ml.100 μl of this suspension was transferred to wells of a 96 well roundbottomed microplate. 100 μl of “Fix & Perm” Medium A (CaltagLaboratories, GAS001 S-100) was added to each well and the plateincubated at room temperature for 15 mins. The cells were pelleted waswashed twice in FACS staining buffer. Following washing, the cells werere-suspended in 50 μl of a solution of the anti-NOGO antibodies or anisotype matched control antibody at a concentration of 2× the finalconcentration (0-200 μg/ml in FACS staining buffer). 50 μl of “Fix &Perm” Medium B (Caltag Laboratories GAS002S-100) was added and the plateincubated on ice for 1 hour. Cells were washed twice in FACS stainingbuffer before being re-suspended in 100 μl of a solution of a PEconjugated anti human γ1 specific goat F(ab′)2 (Sigma P-8047) at adilution of 1/50. Cells incubated for 1 hour on ice. The cells werepelleted and washed 3 times in FACS staining buffer and cellsre-suspended in 100 μl the same buffer. 100 μl of “Fix & Perm” Medium Bwas added to fix the cells. The degree of staining was determined byflow cytometry using a Becton Dickinson FACScan flow cytometer. Theisotype matched controls were used as reference.

Results are shown in FIG. 6 A to F. The totality of the data shownillustrates that the HcLc antibody (of 2A10) gives a strong signal inthis FACS assay, which indicates strong binding to human cell expressedNOGO. The data also shows that humanised versions of this chimera canretain this property. The 2A10 chimeric antibody, and the best humanisedversions, thereof consistently outperform 11C7 in this assay.

TABLE 12 NOGO antibody sequences Summary Sequence identifier (SEQ. I.D.NO) Poly- amino acid nucleotide Description sequence sequence 2A10,CDR-H1 1 — 2A10, CDR-H2 2 — 2A10, CDR-H3 3 — 2A10, CDR-L1 4 — 2A10,CDR-L2 5 — 2A10, CDR-L3 6 — 2A10, VH (murine) 7 41 2A10, VL (murine) 842 Chimeric heavy chain Hc 9 43 Chimeric light chain Lc 10 44 2A10 VHhumanised construct H5 11 45 2A10 VH humanised construct H6 12 46 2A10VH humanised construct H700 13 47 2A10 VH humanised construct H14 14 482A10 VH humanised construct H15 15 49 2A10 VH humanised construct H16 1650 2A10 VH humanised construct H17 17 51 2A10 VH humanised construct H1818 52 2A10 VL humanised construct L6 19 53 2A10 VL humanised constructL13 20 54 2A10 VL humanised construct L14 21 55 2A10 VL humanisedconstruct L15 22 56 2A10 VL humanised construct L16 23 57 2A10 VLhumanised construct L17 24 58 2A10 VL humanised construct L18 25 59 2A10heavy chain humanised construct H5 26 60 2A10 heavy chain humanisedconstruct H6 27 61 2A10 heavy chain humanised construct H700 28 62 2A10heavy chain humanised construct H14 29 63 2A10 heavy chain humanisedconstruct H15 30 64 2A10 heavy chain humanised construct H16 31 65 2A10heavy chain humanised construct H17 32 66 2A10 heavy chain humanisedconstruct H18 33 67 2A10 light chain humanised construct L6 34 68 2A10light chain humanised construct L13 35 69 2A10 light chain humanisedconstruct L14 36 70 2A10 light chain humanised construct L15 37 71 2A10light chain humanised construct L16 38 72 2A10 light chain humanisedconstruct L17 39 73 2A10 light chain humanised construct L18 40 74Campath leader sequence 75 — Amino acids 586-785 of human NOGO A 76 —(NOGO-A56) 2A10 VH humanised construct H1 77 81 2A10 VL humanisedconstruct L11 78 82 2A10 heavy chain humanised construct H1 79 83 2A10light chain humanised construct L11 80 84 2A10 VH humanised constructH19 85 99 2A10 VH humanised construct H20 86 100 2A10 VH humanisedconstruct H21 87 101 2A10 VH humanised construct H22 88 102 2A10 VHhumanised construct H23 89 103 2A10 VH humanised construct H24 90 1042A10 VH humanised construct H25 91 105 2A10 heavy chain humanisedconstruct H19 92 106 2A10 heavy chain humanised construct H20 93 1072A10 heavy chain humanised construct H21 94 108 2A10 heavy chainhumanised construct H22 95 109 2A10 heavy chain humanised construct H2396 110 2A10 heavy chain humanised construct H24 97 111 2A10 heavy chainhumanised construct H25 98 112

1.-30. (canceled)
 31. A humanized monoclonal antibody which binds to anneutralizes human NOGO, the antibody comprising a heavy chain variableregion having an amino acid sequence of SEQ ID NO:86, SEQ ID NO:88, SEQID NO:89 or SEQ ID NO:90, and a light chain variable region having anamino acid sequence of SEQ ID NO:23 or SEQ ID NO:25, or a variantthereof comprising an analog of a complementarity determining region(CDR) of said antibody, wherein said variant retains the bindingspecificity and neutralizing ability of the antibody from which it wasderived.
 32. A monoclonal antibody according to claim 1, comprising VHand VL regions selected from the following list: H24L16 (SEQ ID 86+SEQID 23), H22L16 (SEQ ID 88+SEQ ID 23), H23L16 (SEQ ID 89+SEQ ID 23),H20L18 (SEQ ID 86+SEQ ID 25), H22L18 (SEQ ID 88+SEQ ID 25), H23L18 (SEQID 89+SEQ ID 25), and H24L18 (SEQ ID 90+SEQ ID 25).
 33. A host cellco-transfected with first and second vectors encoding heavy and lightchains, respectively, of an antibody according to claim
 1. 34. A hostcell transfected with a vector encoding heavy and light chains of anantibody according to claim
 1. 35. A method of producing an antibodyaccording to claim 1 or 2, comprising the step of culturing a host cellaccording to claim 3 or 4, and recovering the antibody thereby produced.36. An antibody according to claim 1 or 2 produced by the method ofclaim
 5. 37. A pharmaceutical composition comprising an anti-NOGOantibody or functional fragment thereof according to claim 1, 2 or 6,together with a pharmaceutically acceptable diluent or carrier.
 38. Anantibody according to claim 1, 2, or 6 for use in the treatment ofprophylaxis of a human patient.